ISO 9308-1:2000 PDF

Scope: This part of ISO 9308 describes a reference method (Standard Test) for the detection and enumeration of Escherichia coli and coliform bacteria in water for human consumption. The Standard Test is based on membrane filtration, subsequent culture on a differential agar medium and calculation of the number of target organisms in the sample. The Standard Test has a low selectivity, allowing the detection of injured bacteria. Due to the low selectivity, background growth can interfere with the reliable enumeration of coliform bacteria and E. coli, for example in some drinking waters, like shallow well waters, that have not been disinfected and yield a high background growth. This part of ISO 9308 is therefore especially suitable for disinfected water and other drinking waters of low bacterial numbers. This part of ISO 9308 includes a rapid method (Rapid Test) for the detection of E. coli only within 24 h in water for human consumption, which can be useful in special cases when information is needed quickly. The Rapid Test is based on membrane filtration, subsequent culture under selective conditions and calculation of the number of E. coli in the sample. Standard and Rapid Tests described in this part of ISO 9308 are applicable to other kinds of water provided that suspended matter or background flora does not interfere with filtration, culture and counting.

ISO 9308-1:2000 Referenced Document

ISO 3696:1987 Water for analytical laboratory use; Specification and test methods
ISO 5667-1:1980 Water quality; Sampling; Part 1 : Guidance on the design of sampling programmes
ISO 5667-2:1991 Water quality; sampling; part 2: guidance on sampling techniques
ISO 5667-3:1994 Water quality - Sampling - Part 3: Guidance on the preservation and handling of samples
ISO 6887-1:1999 Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 8199:1988 Water quality; general guide to the enumeration of micro-organisms by culture
ISO/IEC Guide 2 Standardization and related activities - General vocabulary*， 2004-01-01 Update

2016 ISO 9308-1:2014/Amd 1:2016 Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora; Amendment 1
2014 ISO 9308-1:2014 Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora
2007 ISO 9308-1:2000/cor 1:2007 Water quality - Detection and enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method; Technical Corrigendum 1
2000 ISO 9308-1:2000 Water quality - Detection and enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method
1990 ISO 9308-1:1990 Water quality; detection and enumeration of coliform organisms, thermotolerant coliform organisms and presumptive Escherichia coli; part 1: membrane filtration method