ISO 12890:1999 PDF

Scope: This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and wastewaters to embryos and early larval developmental stages of a species of freshwater fish, Danio rerio (Hamilton-Buchanan), Teleostei, Cyprinidae (common name zebrafish). Where necessary this determination may include an acute toxicity test using Danio rerio to determine the 96-h LC50 for zebrafish in accordance with ISO 7346-1, ISO 7346-2 or ISO 7346-3. The method is also applicable to freshwater fish other than zebrafish if appropriate modifications of the test conditions are made, particularly with regard to the temperature and volume per fish biomass.

ISO 12890:1999 Referenced Document

ISO 6341 Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test*， 2012-10-01 Update
ISO 7346-1 Water quality - Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static method
ISO 7346-2 Water quality - Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method
ISO 7346-3 Water quality - Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 3: Flow-through method

1999 ISO 12890:1999 Water quality - Determination of toxicity to embryos and larvae of freshwater fish - Semi-static method