This document specifies the terms and definitions for the determination of graphene quantum dot enzyme activity by UV/visible spectrophotometry, measurement principles, instruments, reagents and equipment, sample pretreatment, measurement conditions and steps, data processing and calculations, uncertainty Source analysis and test reports. This document is suitable for characterizing the enzyme-like activity of graphene quantum dots using UV/visible spectrophotometry. The substrate is o-phenylenediamine and the substrate concentration is 9.74mmol?L-1. The characterization of the enzyme activity of other nanoparticles can also be performed as a reference.