The protein in the milk powder is hydrolyzed by hydrochloric acid into free amino acids. After derivatization with 2,4-dinitrochlorobenzene, it is separated using a C18 chromatographic column, detected by a UV detector, and quantified by an external standard method.
T/CHBAS 14-2021 history
2021T/CHBAS 14-2021 Determination of amino acids content in milk powder High performance liquid chromatography