ASTM E1758-01(2020)
Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography

Standard No.

ASTM E1758-01(2020)

Release Date

2020

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E1758-01(2020)

Scope

1.1 This test method covers the determination of carbohydrates present in a biomass sample, expressed as the percent, by mass, of each sugar on a 105 °C dried mass basis. NOTE 1—The percent sugar must be corrected for the water of hydrolysis before calculating the actual mass percent of the polysaccharide in the original biomass sample. 1.2 Sample materials suitable for this procedure include hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid or alkaline-pretreated biomass (washed free of any residual acid or alkali), and the solid fraction of fermentation residues. All results are reported relative to the 105 °C oven-dried mass of the sample. 1.3 The options for the types of samples to be analyzed in this test method are as follows: 1.3.1 Prepared Biomass Samples: 1.3.1.1 Air Dried (%Tad)—The percent, by mass, of total solids of the air-dried sample. 1.3.1.2 45 °C Dried (%T45)—The percent, by mass, of total solids of the 45 °C dried sample. 1.3.1.3 Freeze Dried (%Tfd)—The percent, by mass, of total solids of the freeze dried sample. 1.3.2 Extractives-Free Sample (%T ext)—The percent, by mass, of total solids of the extracted sample determined at 105°C. 1.4 The values stated in SI units are to be regarded as the standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Note 3 and Note 4. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E1758-01(2020) Referenced Document

• ASTM D1193 Standard Specification for Reagent Water
• ASTM E1690 Standard Test Method for Determination of Ethanol Extractives in Biomass
• ASTM E1721 Standard Test Method for Determination of Acid-Insoluble Residue in Biomass
• ASTM E1756 Standard Test Method for Determination of Total Solids in Biomass
• ASTM E1757 Standard Practice for Preparation of Biomass for Compositional Analysis

ASTM E1758-01(2020) history

• 2020 ASTM E1758-01(2020) Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography
• 2001 ASTM E1758-01(2015) Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography
• 2001 ASTM E1758-01(2007) Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography
• 2001 ASTM E1758-01 Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography
• 1995 ASTM E1758-95e1 Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography