① Feeding of insects ② Use Escherichia coli, deltamethrin, insect saline, ultraviolet rays, and ultrasound as induction sources for induction to obtain hemolymph with antibacterial activity ③ Through the analysis of active components, use the molecular weight, With properties such as isoelectric point, conventional gel chromatography and ion exchange chromatography purification methods are used to obtain electrophoretically pure resistant components. ④ Determination of bactericidal activity of antimicrobial peptides (agar hole method) ⑤ Minimum inhibitory concentration (MIC) of antimicrobial peptides ⑥ Determination of bactericidal kinetics of antimicrobial peptides ⑦ Effect of antimicrobial peptides on cell walls ⑧ Intracellular ultraviolet absorbing substance leakage test