GB/T 41212-2021
Nanotechnologies—5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line (English Version)
- Standard No.
- GB/T 41212-2021
- Language
- Chinese, Available in English version
- Release Date
- 2021
- Published By
- 国家市场监督管理总局、中国国家标准化管理委员会
- Latest
- GB/T 41212-2021
- Scope
- This document describes how to detect and evaluate exposure of RAW264.7 macrophages to nano Methods of generating reactive oxygen species (ROS) after objects, nanoparticles and their aggregates and agglomerates. This document applies to assays using 24-well plates. If other orifice plates are used, it is necessary to adjust the sample volume and verify the effectiveness of the operation steps to ensure the reliability of the test results.
GB/T 41212-2021 Referenced Document
- ISO 10993-5 Biological evaluation of medical devices — Part 5: Tests for cytotoxicity: in vitro methods
GB/T 41212-2021 history
- 2021 GB/T 41212-2021 Nanotechnologies—5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
