T/CAIA SH008-2017
Real-Time Simultaneous Amplification and Testing (SAT) method for detecting S.aureus in foods? (English Version)
- Standard No.
- T/CAIA SH008-2017
- Language
- Chinese, Available in English version
- Release Date
- 2017
- Published By
- Group Standards of the People's Republic of China
- Latest
- T/CAIA SH008-2017
- Scope
- At the same temperature, using bacterial RNA as the starting template, M-MLV reverse transcriptase generates a double-stranded DNA of the target RNA, and then uses T7 RNA polymerase to use this DNA as the template to generate multiple (100 to 1000) RNA copies. ; Each RNA copy number then enters the next amplification cycle starting from reverse transcription; at the same time, after the optimized probe with fluorescent label specifically binds to these RNA copies, it opens the stem-loop structure, thereby generating fluorescence. According to the real-time fluorescence signal The appearance time and intensity of the test result can be determined by combining the positive control and negative control.
T/CAIA SH008-2017 history
- 2017 T/CAIA SH008-2017 Real-Time Simultaneous Amplification and Testing (SAT) method for detecting S.aureus in foods?
