BS EN 16923:2022 Foodstuffs. Determination of T-2 toxin and HT-2 toxin in cereals and cereal products for infants and young children by SPE clean up and HPLC-MS/MS
1 Scope
This document describes a method for the determination of T-2 toxin and HT-2 toxin
in cereals and cereal-based products, e.g. oats, intended for nutrition of infants
and young children by high performance liquid chromatography (HPLC) coupled with tandem
mass spectrometry (MS/MS) after cleanup by solid phase extraction (SPE) [5].
The method has been validated for HT-2 toxin in oat flour at levels of 9,3 µg/kg and
28,1 µg/kg, oat flakes at levels of 16,5 µg/kg and 21,4 µg/kg, and breakfast cereals
(containing oat flakes) at a level of 8,1 µg/kg and for T-2 toxin in oat flour at
levels of 4,4 µg/kg and 8,3 µg/kg, oat flakes at levels of 4,9 µg/kg and 6,6 µg/kg
and breakfast cereals (containing oat flakes) at a level of 3,5 µg/kg.
Laboratory experiences [6] have shown that the method is also applicable to highly swelling materials (dry
cereal-based porridges and modified starches), but these were not examined in the
method validation study. Details are outlined in 7.3.
The method can also be applied to oat-by-products at higher levels of T-2- and HT-2
toxin. In this case, the dilution steps need to be considered [6].
The method can also be applied to cereals and cereal products for infants and young
children based on e.g. wheat, barley and rice. In this case, the method needs to be
in-house-validated for each material. At the time of the interlaboratory study, planned
range was 10 µg/kg to 100 µg/kg, and it is known from the pre-study that the method
works well in the whole range, although final validation was only done in the range
from 3,5 µg/kg to 28,1 µg/kg.
BS EN 16923:2022 Referenced Document
EN ISO 3696 Water for Analytical Laboratory Use- Specification and Test Methods (ISO 3696 : 1987)
BS EN 16923:2022 history
2022BS EN 16923:2022 Foodstuffs. Determination of T-2 toxin and HT-2 toxin in cereals and cereal products for infants and young children by SPE clean up and HPLC-MS/MS
2017BS EN 16923:2017 Foodstuffs. Determination of T-2 toxin and HT-2 toxin in cereals and cereal products for infants and young children by LC-MS/MS after SPE cleanup