ASTM D8238-18(2023) PDF

Scope: 1.1 This test method covers an immunological method to determine the amount of protein in Guayule Natural Rubber (GNR) and its products using rabbit antisera specific for Guayule Natural Rubber latex proteins. This immunoassay procedure quantitatively measures the level of GNR proteins in solution using an inhibition format. The samples may include but are not restricted to, gloves or other GNR product extracts, and liquid GNR samples which have been collected in order to measure the protein levels. 1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D8238-18(2023) Referenced Document

ASTM D5712 Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
ASTM D6499 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products
ASTM D7427 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex*， 2024-04-21 Update
ASTM E177 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
ASTM E691 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method

2023 ASTM D8238-18(2023) Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins
2018 ASTM D8238-18 Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins