T/BTFDIA T15/BTFDIA002-2019
Rapid detection method for pathogenic bacteria in non-prepackaged ready-to-eat food (English Version)

Standard No.
T/BTFDIA T15/BTFDIA002-2019
Language
Chinese, Available in English version
Release Date
2020
Published By
Group Standards of the People's Republic of China
Latest
T/BTFDIA T15/BTFDIA002-2019
Scope
The pathogenic microorganisms in the sample are enriched and cultured, and then the nucleic acid is extracted. The DNA fragment of the pathogenic microorganism is used as a template. After amplifying the primers labeled with biotin (digoxigenin) at both ends, the amplified sample liquid is fixed with The membranes of streptavidin (digoxigenin) and colloidal gold flow together downward. When they flow to the detection line area coated with the corresponding antibody, the biotin (digoxigenin)-labeled antigen in the amplification sample solution reacts with Streptavidin (digoxigenin), colloidal gold and corresponding antibodies undergo a specific binding reaction, causing a certain color to appear at the detection line, thereby achieving specific immunodiagnosis.

T/BTFDIA T15/BTFDIA002-2019 history

Rapid detection method for pathogenic bacteria in non-prepackaged ready-to-eat food



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