The pathogenic microorganisms in the sample are enriched and cultured, and then the nucleic acid is extracted. The DNA fragment of the pathogenic microorganism is used as a template. After amplifying the primers labeled with biotin (digoxigenin) at both ends, the amplified sample liquid is fixed with The membranes of streptavidin (digoxigenin) and colloidal gold flow together downward. When they flow to the detection line area coated with the corresponding antibody, the biotin (digoxigenin)-labeled antigen in the amplification sample solution reacts with Streptavidin (digoxigenin), colloidal gold and corresponding antibodies undergo a specific binding reaction, causing a certain color to appear at the detection line, thereby achieving specific immunodiagnosis.
T/BTFDIA T15/BTFDIA002-2019 history
2020T/BTFDIA T15/BTFDIA002-2019 Rapid detection method for pathogenic bacteria in non-prepackaged ready-to-eat food