ASTM D6503-19
Standard Test Method for Enterococci in Water Using Enterolert

Standard No.

ASTM D6503-19

Release Date

2019

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM D6503-24

Latest

ASTM D6503-24

Scope

1.1 This test method covers a simple procedure for the detection of enterococci in water and wastewater. It is based on IDEXX’s patented Defined Substrate Technology (DST).2 This product, Enterolert, utilizes a nutrient indicator that fluoresces when metabolized. It can detect these bacteria at one most probable number (MPN)/100 mL within 24 h. The presence of this microorganism in water is an indication of fecal contamination and the possible presence of enteric pathogens. 1.2 This test method can be used successfully with drinking water, source water, recreational (fresh and marine) water, wastewater, and bottled water. It is the user’s responsibility to ensure the validity of this test method for waters of untested matrices. 1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D6503-19 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1193 Standard Specification for Reagent Water
• ASTM D2777 Standard Practice for Determination of Precision and Bias of Applicable Methods of Committee D-19 on Water
• ASTM D3370 Standard Practices for Sampling Water from Closed Conduits

ASTM D6503-19 history

• 2024 ASTM D6503-24 Standard Test Method for Enterococci in Water Using Enterolert
• 2019 ASTM D6503-19 Standard Test Method for Enterococci in Water Using Enterolert
• 2014 ASTM D6503-14 Standard Test Method for Enterococci in Water Using Enterolert
• 1999 ASTM D6503-99(2009) Standard Test Method for Enterococci in Water Using Enterolert
• 1999 ASTM D6503-99(2005) Standard Test Method for Enterococci in Water Using Enterolert
• 1999 ASTM D6503-99 Standard Test Method for Enterococci in Water Using Enterolert_TM