T/SDAQI 007-2021
Rapid qualitative detection of Pseudomonas aeruginosa in production water Real-time PCR method (English Version)
- Standard No.
- T/SDAQI 007-2021
- Language
- Chinese, Available in English version
- Release Date
- 2021
- Published By
- Group Standards of the People's Republic of China
- Latest
- T/SDAQI 007-2021
- Scope
- After the sample is filtered by the suction filtration equipment, the filtered membrane is placed in the selective medium for culture. The cultured bacterial fluid was collected for DNA extraction. Using DNA as the template and the bacterial 16SrDNA gene sequence as the internal reference, relatively conserved and highly specific primers and probes from the exotoxin A (eta) gene in Pseudomonas aeruginosa were used to perform real-time fluorescence PCR amplification of the target. According to Ct value to determine whether the sample contains Pseudomonas aeruginosa. Among them, the detection of internal reference reaction can monitor whether the reaction is proceeding normally and prevent false negative results.
T/SDAQI 007-2021 history
- 2021 T/SDAQI 007-2021 Rapid qualitative detection of Pseudomonas aeruginosa in production water Real-time PCR method
