ASTM D8429-21
Standard Test Method for Legionella pneumophila in Water Samples Using Legiolert

Standard No.

ASTM D8429-21

Release Date

2021

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM D8429-21

Scope

1.1 This test method describes a simple procedure for the detection of Legionella pneumophila (L. pneumophila) in potable water and non-potable waters (cooling towers, for example). This procedure describes a liquid culture method based on a bacterial enzyme technology. The detection of L. pneumophila is signaled through the utilization of a substrate present in the Legiolert reagent. L. pneumophila cells grow rapidly and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the Legiolert reagent. Actively growing strains of L. pneumophila use the added substrate to produce a brown color indicator or produce turbid growth with or without brown coloration. Legiolert can detect this bacterial species at the following minimum concentrations based on the protocol employed: 1.1.1 Potable Water: 1.1.1.1 ≥1 organism / 100 mL at 7 days for 100 mL potable protocol. 1.1.1.2 ≥1 organism / 10 mL at 7 days for 10 mL potable protocol. 1.1.2 Non-potable Water: 1.1.2.1 ≥1 organism / 1.0 mL at 7 days for 1.0 mL non-potable protocol. 1.1.2.2 ≥1 organism / 0.1 mL at 7 days for 0.1 mL non-potable protocol. 1.1.3 This test method can be used for potable (drinking) waters and non-potable waters such as cooling tower waters (1).3 It is the user’s responsibility to ensure the validity of this test method for waters of untested matrices. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D8429-21 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1193 Standard Specification for Reagent Water
• ASTM D2777 Standard Practice for Determination of Precision and Bias of Applicable Methods of Committee D-19 on Water
• ASTM D3370 Standard Practices for Sampling Water from Closed Conduits
• ASTM D5952 Standard Guide for Inspecting Water Systems for Legionellae and Investigating Possible Outbreaks of Legionellosis (Legionnaires' Disease or Pontiac Fever)
• ASTM D6503 Standard Test Method for Enterococci in Water Using Enterolert

ASTM D8429-21 history

• 2021 ASTM D8429-21 Standard Test Method for Legionella pneumophila in Water Samples Using Legiolert