T/CAIA SHO19-2022
Acetylcholinesterase-Determination of activity -Spectrophotometric method (English Version)
- Standard No.
- T/CAIA SHO19-2022
- Language
- Chinese, Available in English version
- Release Date
- 2022
- Published By
- Group Standards of the People's Republic of China
- Latest
- T/CAIA SHO19-2022
- Scope
- Acetylcholinesterase hydrolyzes thioacetylcholine iodide to produce thiocholine and acetic acid. Thiocholine reacts with the mercapto chromogen 5, 5'-dithiobis(2-nitrobenzoic acid) (DTNB) to form a yellow compound 5-mercapto-2-nitrobenzoic acid (TNB). TNB is at wavelength There is a characteristic light absorption at 412 nm, and the absorbance value is measured; according to the standard solution prepared by reduced glutathione and the reaction with DTNB, the standard curve of the relationship between the amount of glutathione and the absorbance is drawn, and the enzyme of acetylcholinesterase is calculated. activity value.
T/CAIA SHO19-2022 history
- 2022 T/CAIA SHO19-2022 Acetylcholinesterase-Determination of activity -Spectrophotometric method
