This document specifies multiplex RT-PCR and multiplex qRT-PCR methods for the identification and detection of African swine fever virus, swine fever virus and swine SARS virus, including principles, test conditions, reagents, main instruments and equipment, sample collection and processing , multiplex RT-PCR detection, multiplex qRT-PCR detection, negative and positive controls and result determination and other technical requirements. This document is applicable to the detection of African swine fever virus, swine fever virus and swine SARS virus nucleic acid.
T/GXAS 489-2023 history
2023T/GXAS 489-2023 Establishment of multiplex RT-PCR and multiplex qRT-PCR for differential detection of ASFV, CSFV and APPV