GB/T 39228-2020 (English Version) PDF

Standard No.: GB/T 39228-2020
Language: Chinese, Available in English version
Release Date: 2020
Published By: General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
Latest: GB/T 39228-2020

Scope: This standard specifies a method for estimating soil microbial biomass by measuring the total amount of extractable organic matter after killing fresh soil microorganisms by fumigation with chloroform. This standard only describes the determination of extractable organic carbon content, but this method is also applicable to the estimation of microbial nitrogen content and microbial ninhydrin reaction nitrogen content in soil. This standard applies to the determination of microbial biomass in aerobic and anaerobic (waterlogged, paddy fields) soils with a wide range of soil pH. This standard is also applicable to the determination of microbial biomass in supersaturated soil containing easily decomposable organic substrates and potassium sulfate solution. NOTE: Chloroform fumigation also affects soil fauna, but the carbon contribution of such organisms is generally small (<5%) and usually negligible.

GB/T 39228-2020 Referenced Document

ISO 10381-6:1993 Soil quality; sampling; part 6: guidance on the collection, handling and storage of soil for the assessment of aerobic microbial processes in the laboratory
ISO 10694:1995 Soil quality - Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11465:1993 Soil quality; determination of dry matter and water content on a mass basis; gravimetric method

2020 GB/T 39228-2020 Determination of soil microbial biomass—Fumigation-extraction method