ASTM E3371-22
Standard Test Method for Measuring the Ability of a Synthetic Polymeric Material to Resist Bacterial Adherence

Standard No.

ASTM E3371-22

Release Date

2022

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E3371-22

Scope

1.1 This method is designed to evaluate (quantitatively) the number of bacteria attached to the flat, two-dimensional surfaces of synthetic polymeric materials and polymeric coatings on various substrates that may or may not contain bound or incorporated anti-adherent agents. The method focuses on assessing the ability of the surface to reduce bacterial attachment. Other microorganisms such as yeast and fungal conidia may be tested using this method. 1.2 This test method quantitatively determines the differences in bacterial adherence seen between synthetic polymeric surfaces that allow bacterial adherence and those that do not, comparing the number of organisms recovered from the control surface to the number recovered from the test specimen surface after the contact time. Knowledge of microbiological techniques is required for these procedures. 1.3 This test method specifies proper methods for measuring the ability of a synthetic polymeric material to resist adherence against specified organism. Due to individual sensitivities, the result of one test organism might not be applicable for other organisms. 1.4 This test method is designed to measure the potential ability to resist bacterial adherence of a non-porous surface compared directly to a polyester control panel known to support bacterial adherence under specific testing conditions. 1.5 Antimicrobial treated non-porous surfaces may demonstrate ability to resist bacterial adherence in this method. This method does not purport to differentiate between antiadherence and antimicrobial activity nor is it designed to reflect specific end-use or environmental conditions. Any product that demonstrates ability to resist bacterial adherence in this method should be measured for antimicrobial activity using a separate test technique such as Test Method E2180 or ISO 22196. 1.6 The method focuses on assessing the ability of synthetic polymeric materials and polymeric coatings on various substrates to reduce bacterial attachment. The specimen with absorbing or adhesive surfaces may be unable to be disinfected properly before testing, or may trap inoculated organism during recovery process and thus lead to a false result. This method does not apply to specimens with absorbent or adhesive surfaces. 1.7 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 This test method is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of Subcommittee E35.15 on Antimicrobial Agents. Current edition approved Oct. 1, 2022. Published November 2022. DOI: 10.1520/E3371–22. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 2. Referenced Documents

ASTM E3371-22 Referenced Document

• ASTM E177 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
• ASTM E2180 Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials*， 2024-04-01 Update
• ASTM E2756 Standard Terminology Relating to Antimicrobial and Antiviral Agents
• ASTM E3151 Standard Test Method for Determining Antimicrobial Activity and Biofilm Resistance Properties of Tube, Yarn, or Fiber Specimens
• ISO 22196 Measurement of antibacterial activity on plastics and other non-porous surfaces

ASTM E3371-22 history

• 2022 ASTM E3371-22 Standard Test Method for Measuring the Ability of a Synthetic Polymeric Material to Resist Bacterial Adherence