This document specifies the reagents and consumables, instruments and equipment, samples, test steps, and test data processing for real-time fluorescence PCR of animal Brucella (Brucellaspp.). This document is mainly used for nucleic acid detection of Brucella (Brucellaspp.). The samples mainly include: animal spleen, liver, lymph node, blood, milk sample, semen, vaginal secretions, aborted fetal contents, and can also detect Brucella. Contaminated environmental samples.