This document uses PCR technology to extract DNA from mushroom samples. After PCR amplification, electrophoresis, cloning and sequencing, it is compared with the gene sequence in the existing database to identify the species of the sample. The experimental reagents, consumables, instruments and equipment involved are all commonly used laboratory equipment and can meet the identification needs of grassroots units. The PCR amplification step sets up blank controls, positive controls, and negative controls as quality controls to avoid possible false positives and false negatives in identification results.
T/CIS 67002-2021 history
2021T/CIS 67002-2021 Species identification of three lethal amanitas—PCR amplification - Sanger sequencing