ASTM D8243-19
Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method

Standard No.

ASTM D8243-19

Release Date

2019

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM D8243-19

Scope

1.1 This test method provides a protocol for using enzymelinked immunosorbent assay (ELISA) technology to test water samples for the enzyme adenosine 5’-phosphosulfate reductase (APSr) concentration. 1.1.1 APSr is present in all known sulfate reducing protists (SRP – sulfate reducing bacteria – SRB – and sulfate reducing archaea – SRA). 1.1.2 As reported in U.S. Patent 4,999,286, APS reductase concentration can be used as a surrogate parameter for estimating SRA bioburdens (Appendix X1 compares results from Test Methods D8243, D4412, and quantitative polymerase chain reaction – qPCR – testing). 1.2 This test method has been validated in tap water, oilfield produced water (salinities ranging from 100 g L-1 to 600 g L-1 ), and fuel-associated water (commonly referred to as bottomswater). 1.3 This test method detects APS reductase semiquantitatively in the range of 0.001M to 0.1M – correlating to 102 SRP/mL to 106 SRP/mL. 1.3.1 As described in Appendix X2 test method sensitivity can be increased 10-fold to 100-fold. However, the precision statistics provided in X apply only to 10-mL specimens. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Some specific hazards statements are given in Section 9 on Hazards. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D8243-19 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D4412 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
• ASTM D5847 Standard Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
• ASTM D6300 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
• ASTM D6499 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products*， 2023-05-01 Update
• ASTM D7464 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing*， 2020-05-01 Update
• ASTM E1601 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
• ASTM E1847 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
• ASTM E1914 Standard Practice for Use of Terms Relating to the Development and Evaluation of Methods for Chemical Analysis
• ASTM E691 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method

ASTM D8243-19 history

• 2019 ASTM D8243-19 Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method