1.1 This test method delivers a protocol for a quantitative measure of nitrite (NO2 – ), a stable end-product of nitric oxide (NO), in cell culture medium due to exposure to nanomaterial(s).
1.2 NO has a critical role in several pathological conditions in addition to its role in many physiological processes.
1.3 This test method uses murine macrophage cell line RAW 264.7 as an in vitro model.
1.4 The nitrite is measured in the cell culture medium by a colorimetric analysis using Griess reagent as shown in Fig. 1.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM E3351-22 Referenced Document
ASTM E2490 Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Photon Correlation Spectroscopy (PCS)
ASTM E2834 Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA)*, 2022-09-01 Update
ASTM F1877 Standard Practice for Characterization of Particles
ASTM F1903 Standard Practice for Testing for Biological Responses to Particles In Vitro
ASTM E3351-22 history
2022ASTM E3351-22 Standard Test Method for Detection of Nitric Oxide Production In Vitro