ASTM E925-09(2022)
Standard Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Bandwidth does not Exceed 2 nm

Standard No.

ASTM E925-09(2022)

Release Date

2022

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E925-09(2022)

Scope

1.1 This practice covers the parameters of spectrophotometric performance that are critical for testing the adequacy of instrumentation for most routine tests and methods2 within the wavelength range of 200 nm to 700 nm and the absorbance range of 0 to 2. The recommended tests provide a measurement of the important parameters controlling results in spectrophotometric methods, but it is specifically not to be inferred that all factors in instrument performance are measured. 1.2 This practice may be used as a significant test of the performance of instruments for which the spectral bandwidth does not exceed 2 nm and for which the manufacturer’s specifications for wavelength and absorbance accuracy do not exceed the performance tolerances employed here. This practice employs an illustrative tolerance of 61 % relative for the error of the absorbance scale over the range of 0.2 to 2.0, and of 61.0 nm for the error of the wavelength scale. A suggested maximum stray radiant power ratio of 4 × 10-4 yields <1 % absorbance bias at an absorbance of 2. These tolerances are chosen to be compatible with many chemical applications while comfortably exceeding the uncertainty of the certified values for the reference materials and typical manufacturer’s specifications for error in the wavelength and absorbance scales of the instrument under test. The user is encouraged to develop and use tolerance values more appropriate to the requirements of the end use application. This procedure is designed to verify quantitative performance on an ongoing 1 This practice is under the jurisdiction of ASTM Committee E13 on Molecular Spectroscopy and Separation Science and is the direct responsibility of Subcommittee E13.01 on Ultra-Violet, Visible, and Luminescence Spectroscopy. Current edition approved Nov. 1, 2022. Published November 2022. Originally approved in 1983. Last previous edition approved in 2014 as E925 – 09 (2014). DOI: 10.1520/E0925-09R22. 2 Routine tests are defined as those in which absorbance data obtained on a sample are compared to those of a standard sample preparation. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 basis and to compare one instrument’s performance with that of other similar units. Refer to Practice E275 to extensively evaluate the performance of an instrument. 1.3 This practice should be performed on a periodic basis, the frequency of which depends on the physical environment within which the instrumentation is used. Thus, units handled roughly or used under adverse conditions (exposed to dust, chemical vapors, vibrations, or combinations thereof) should be tested more frequently than those not exposed to such conditions. This practice should also be performed after any significant repairs are made on a unit, such as those involving the optics, detector, or radiant energy source. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E925-09(2022) Referenced Document

• ASTM E131 Standard Definitions of Terms and Symbols Relating to Molecular Spectroscopy
• ASTM E169 Standard Practices for General Techniques of Ultraviolet-Visible Quantitative Analysis
• ASTM E1866 Standard Guide for Establishing Spectrophotometer Performance Tests
• ASTM E275 Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
• ASTM E387 Standard Test Method for Estimating Stray Radiant Power Ratio of Spectrophotometers by the Opaque Filter Method
• ISO/IEC 17025 General requirements for the competence of testing and calibration laboratories [Standard in French]

ASTM E925-09(2022) history

• 2022 ASTM E925-09(2022) Standard Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Bandwidth does not Exceed 2 nm
• 2009 ASTM E925-09(2014) Standard Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Bandwidth does not Exceed 2 nm
• 2009 ASTM E925-09 Standard Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Slit Width does not Exceed 2 nm
• 2002 ASTM E925-02 Standard Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Slit Width does not Exceed 2 nm
• 2002 ASTM E925-83(1994)e1 Standard Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Slit Width does not Exceed 2 nm