BS EN 17270:2019
Animal feeding stuffs: Methods of sampling and analysis. Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography
- Standard No.
- BS EN 17270:2019
- Release Date
- 2019
- Published By
- British Standards Institution (BSI)
- Latest
- BS EN 17270:2019
- Scope
- What is BS EN 17270- D etermination of theobromine in animal feeding stuffs about?
BS EN 17270 is a standard that covers the determination of theobromine in animal feeding stuffs that includes cocoa derived ingredients that enable limiting of the contamination and toxicity in animal feeds.
BS EN 17270 specifies a test method for the determination of theobromine in feed material or compound feed in the working range 27 mg/kg to 307 mg/kg using liquid chromatography coupled to a UV detector (HPLC-UV) or in the working range 49 mg/kg to 307 mg/kg using liquid chromatography with tandem mass spectrometry (LC-MS/MS).
BS EN 17270 method has been fully validated using complementary compound feed for adult dogs and complementary compound feed for horses.
BS EN 17270 method is also considered applicable for determining theobromine in baking chocolate using either HPLC-UV or LC-MS/MS systems. The working range can be extended provided the extended range is validated. BS EN 17270:2019 history
- 2019 BS EN 17270:2019 Animal feeding stuffs: Methods of sampling and analysis. Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography
