This document specifies a method for the determination of free gossypol, extractable by acidified acetonitrile/water, in cottonseed, cottonseed products and compound feeds by liquid chromatography with tandem mass spectrometry (LC-MS/MS).
The method described in this document has been successfully validated in the range of 69 mg/kg to 5 950 mg/kg by collaborative trial in the following matrices: cottonseed, cottonseed products (cake/meal, hulls) and compound feeds for bovine, porcine and poultry.
might be applicable at lower and at higher concentrations than the concentration range validated in the collaborative trial. However, this needs to be assessed by in-house validation.
BS EN 17504:2022 Referenced Document
EN ISO 3696 Water for Analytical Laboratory Use- Specification and Test Methods (ISO 3696 : 1987)
BS EN 17504:2022 history
2022BS EN 17504:2022 Animal feeding stuffs: Methods of sampling and analysis. Determination of gossypol in cotton seed and feeding stuff by LC-MS/MS